UV/VUS/NIR absorbance spectroscopy is governed by Beer's Law, where the absorbance signal is proportional to chemical concentration, light path length and the compound's specific molar absorbance coefficient. Optical pathlengths of typcial cuvettes and flow cells are between 0.1 cm and 10cm. Longer pathlengths are difficult to achieve due to mechanical constraints. Liquid Waveguide Capillary Cells (LWCC's) fill this gap.
- LWCC's are fiber optic flow cells that combine an increased optical pathlength (10-500cm) with small sample volumes ranging from 2.4uL to about 3mL. Compared to a standard 1 cm cuvette, a 1mAU signal is enhanced one hundred fold with a 100cm flow cell to 100mAU, using WPI's patented waveguide technology. WPI’s Liquid Waveguide Capillary Cells are made of fused silica tubing with an outer coating of a low refractive index polymer. Your liquid sample is guided through the capillary and represents the core of the waveguide.
- Low Volume Fiber Optic Flow Cell for UV/VIS/NIR absorbance analysis. Based on WPI’s established liquid core waveguide technology, the analyte solution functions as the core of a fluid filled light waveguide. Wetted parts in the sample cell light path are PEEK, fused silica and PTFE. Optical fibers are used to transport light to and from the sample cell. The cell can be used in biochemistry for DNA, RNA & protein quantification, colorimetric nutrient and trace metal analysis, drug discovery and dissolution testing, process control, and HPLC analysis.
- A high performance fiber optic spectrophtometer with a unique multiple pathlength sampe cell for absorbance spectroscopy. The multi-pathlength cell, UltraPath has four optical pathlengths contained within offering flexibility for the user to dial in the pathlength suitable for the project on hand. There are four pathlengths to selectf offering a very high senstivity and an extended dynamic range for UV and VIS absorbance measurements. The fluid path of the sample cell is optimized to produce a laminar flow that is virtually free of interference from trapped air bubbles and adherence of dissolved substances to the cell wall.